題目：采用任意點(diǎn)擴(kuò)展函數(shù)進(jìn)行快速、魯棒、精確的三維定位
作者：Yiming Li, Markus Mund, Philipp Hoess, Ulf Matti, Bianca Nijmeijer, Vilma Jimenez Sabinina,Jan Ellenberg, Ingmar Schoen, Jonas Ries
European Molecular Biology Laboratory (EMBL), Cell Biology and Biophysics unit,Meyerhofstr. 1, 69117 Heidelberg, Germany
Royal College of Surgeons in Ireland, 123 St. Stephen's Green, Dublin 2, Ireland
Correspondence should be addressed to J.R. (jonas.ries@embl.de)
Abstract
We present a fitter for 3D single-molecule localization of arbitrary, experimental point spread functions (PSFs) that reaches minimum uncertainty for EMCCD and sCMOS cameras, and achieves more than 10^5 fits/s. We provide tools to robustly model experimental PSFs and correct for depth induced aberrations, which allowed us to achieve an unprecedented 3D resolution with engineered astigmatic PSFs, and acquire high quality 3D superresolution images even on standard microscopes without 3D optics.
提出了一種適用于EMCCD和sCMOS攝像機(jī)的任意、實(shí)驗(yàn)點(diǎn)擴(kuò)展函數(shù)(PSFs)的三維單分子定位的方法，達(dá)到了10^5的擬合/秒。我們提供的工具是對模型實(shí)驗(yàn)的PSFs和正確的深度誘發(fā)象差，這使我們能夠達(dá)到前所未有的三維分辨率的工程散光PSFs，并獲得高質(zhì)量的三維超分辨率圖像，即使在標(biāo)準(zhǔn)顯微鏡，沒有三維光學(xué)。
本論文預(yù)覽版詳細(xì)內(nèi)容：Fast, robust and precise 3D localization for arbitrary point spread functions.pdf
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